Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3752-3757, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-346844
ABSTRACT
<p><b>OBJECTIVE</b>To identify SNP in flos Lonicerae, and authenticate Lonicera japonica from its adulterants and the mixture by using bidirectional PCR amplification of specific alleles (Bi-PASA).</p><p><b>METHOD</b>SNP of L. japonica and its adulterants was identified by using ClustulW to align trnL-trnF sequences of the Lonicera genus from GenBank database. Bi-PASA primer was designed and the PCR reaction systems including annealing temperature optimized. Optimized result was performed in 84 samples to authenticate L. japonica, its adulterants and the mixture.</p><p><b>RESULT</b>When the annealing temperature was 61 degrees C, DNA from L. japonica would be amplified 468 bp whereas PCR products from all of the 9 adulterants were 324 bp. The established method also can detect 5% of intentional adulteration DNA into L. japonica.</p><p><b>CONCLUSION</b>The Bi-SPASA could authenticate L. japonica from its adulterants and the mixture.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Especificidade da Espécie
/
RNA de Transferência de Leucina
/
RNA de Transferência de Fenilalanina
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Classificação
/
DNA de Plantas
/
Polimorfismo de Nucleotídeo Único
/
Lonicera
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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