PCR-SSCP molecular identification of Panax ginseng and P. quinquefolius based on ITS2 bar coding SNPs / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3748-3751, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-346845
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for identifying Panax ginseng and P. quinquefolius with PCR-SSCP, on the basis of specific SNP identification sites of their ITS2 bar codes.</p><p><b>METHOD</b>ITS2 sequences of P. ginseng and P. quinquefolius recorded in GenBank were searched to conduct a comparative analysis and screen out specific SNP identification sites of their ITS2 bar codes. Based on that, the Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method was adopted for analyzing 11 P. ginseng samples and 10 P. quinquefolius samples and verifying sequencing of their PCR products.</p><p><b>RESULT</b>The P. ginseng and P. quinquefolius samples had the same agarose mages of PCR-SSCP with the standard medicines. There were significant differences between the PCR-SSCP agarose mages of P. ginseng and P. quinquefolius, with identifical identification results between PCR-SSCP and sequencing.</p><p><b>CONCLUSION</b>Compared with the sequencing method, PCR-SSCP is so rapid and accurate that it can be used for identifying P. ginseng and P. quinquefolius medicines.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade da Espécie
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Classificação
/
Polimorfismo Conformacional de Fita Simples
/
DNA de Plantas
/
Polimorfismo de Nucleotídeo Único
/
DNA Espaçador Ribossômico
/
Eletroforese em Gel de Ágar
/
Código de Barras de DNA Taxonômico
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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