Improvement of carbon tetrachloride drug-induced liver injury model in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3633-3636, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-346893
ABSTRACT
<p><b>OBJECTIVE</b>To establish an ideal CCl4 drug-induced liver injury model in vitro.</p><p><b>METHOD</b>Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity.</p><p><b>RESULT</b>The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect.</p><p><b>CONCLUSION</b>The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspartato Aminotransferases
/
Superóxido Dismutase
/
Ferimentos e Lesões
/
Tetracloreto de Carbono
/
Alanina Transaminase
/
Células Hep G2
/
Doença Hepática Induzida por Substâncias e Drogas
/
Fígado
/
Metabolismo
/
Modelos Biológicos
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS