Regulatory effect of berberine on unbalanced expressions of renal tissue TGF-beta1/SnoN and smad signaling pathway in rats with early diabetic nephropathy / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3604-3610, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-346898
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of berberine (BBR) on unbalanced expression of renal tissue TGF-beta1/SnoN and Smad signal pathway in rats with early diabetic nephropathy (DN), and discuss BBR's effect on DN rats with early diabetic nephropathy and its possible mechanism.</p><p><b>METHOD</b>DN rat model were established by injecting streptozotocin (STZ). The rats were divided into six groups the control group, the model group, three BBR (50, 100, 200 mg x kg(-1)) treatment groups and the enalapril treatment group. They were orally administered once a day for five weeks. The fasting blood glucose (FBG), blood urea nitrogen (BUN), serum creatinine (Scr), urinary protein (24 h Upro) and urinary microalbumin (24 h UmAlb) were tested. The pathological changes in renal tissues were examined by optical microscopy. Immunohistochemical measures were used to detect the expressions of TGF-beta1, SnoN, Smad2/3 and Smad7 protein, and RT-PCR was used to detect TGF-beta1 mRNA in renal tissues.</p><p><b>RESULT</b>Compared with the model group, BBR-treated groups showed significant decrease in FBG, BUN, Scr, 24 h Upro, 24 h UmAlb, TGF-beta1 protein, mRNA and Smad2/3 protein, abnormal morphological improvement in renal tissues, and notable increase in the expressions of SnoN and Smad7 protein.</p><p><b>CONCLUSION</b>BBR can maintain the dynamic balance in TGF-beta1/SnoN expression in renal tissues through Smad signaling pathway, so as to mitigate renal functional disorder in DN rats and delay DN and its development.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Transcrição
/
Berberina
/
Transdução de Sinais
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Expressão Gênica
/
Ratos Sprague-Dawley
/
Nefropatias Diabéticas
/
Tratamento Farmacológico
/
Proteínas Smad
/
Fator de Crescimento Transformador beta1
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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