Protective effects of resveratrol on neonatal rat cardiomyocyte lesion induced by hypoxia / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 94-98, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-346938
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of resveratrol (Res) on neonatal rat cardiomyocyte lesion induced by hypoxia.</p><p><b>METHOD</b>The cardiomyocyte of neonatal rats were cultured in vitro and the model of cardiomyocyte hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay, the HIF-1alpha expression levels in myocardial cells was detected by immunohistochemical, the activities of peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured as well.</p><p><b>RESULT</b>After the administration of hypoxia for 24 hours, the HIF-1alpha expression in myocardial cells was significantly increased. The LDH level in the culture medium was increased from (93.07 +/- 15.84) U x L(-1) to (750.77 +/- 181.51) U x L(-1) (P < 0.01). The intracellular GSH-Px activity was decreased from (46.96 +/- 8.36) U x mL(-1) to (27.13 +/- 4.76) U x mL(-1) (P < 0.05). Res 25, 50 and 75 micromol x L(-1) could dose-dependently inhibit the raising of the HIF-1alpha expression in myocardial cells induced by hypoxia. The LDH activities were decreased dose-dependently to (486.17 +/- 69.97), (189.43 +/- 32.07), (155.34 +/- 29.57) U x L(-1), respectively (P <0.05 or P <0.01). The GSH-Px activities were increased dose-dependently (33.55 +/- 6.34), (37.67 +/- 6.73), (41.44 +/- 7.91) U x mL(-1) (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>Res has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Estilbenos
/
Hipóxia Celular
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Miócitos Cardíacos
/
Relação Dose-Resposta a Droga
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
L-Lactato Desidrogenase
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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