Pressurized liquid extraction of Tripterygiumw wilfordii polyglycosides and optimization of extraction conditions / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the extraction method of polyglycosides from Tripterygium wilfordii.</p><p><b>METHOD</b>The extraction method of pressurized liquid extraction was employed and chromogenic colorimetric technique were used for quantitative analysis. Based on the single-factor experiment, according to the center combination design this paper used three factors and three levels of response surface methodology for process optimization.</p><p><b>RESULT</b>The optimized conditions were as follows ratio of solid to liquid was 19.5, at the temperature of 115 degrees C for 80 minutes, the actual extract ratio and purity of polyglycosides obtained were 0.21% and 0.52%, respectively.</p><p><b>CONCLUSION</b>The method of pressurized liquid extraction has obvious advantages over conventional reflux extracting.</p>