Determination of chelerythrine in Chelidonium majus by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2918-2919, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-346998
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.</p><p><b>METHOD</b>Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (2575) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.</p><p><b>RESULT</b>The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.</p><p><b>CONCLUSION</b>The method is accurate, sensitive and reliable.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Chelidonium
/
Benzofenantridinas
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Artigo
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