Effects of lead exposure on acid-sensing ion channel in hippocampus of baby-rats / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 729-732, 2008.
Article
em Zh
| WPRIM
| ID: wpr-347229
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of chronic lead exposure on mRNA and protein expression of ASIC1a, ASIC2a, ASIC2b in hippocampus of baby-rats.</p><p><b>METHODS</b>The Wistar pregnant rats were randomly divided into 3 groups fed with distilled water or lead contained water (0.2% and 1.0% lead acetate) respectively, 5 rats in each group. The lead-exposure ranged from the 0 day of pregnancy to the offspring weaned. Then the baby-rats were fed with lead water like their mothers and killed at postnatal day 8 or 50. Atomic absorption spectrometry was used to determine lead content in the brain. RT-PCR and Western blotting were used to observe mRNA and protein expression of ASIC1a, ASIC2a and ASIC2b in their hippocampus respectively.</p><p><b>RESULTS</b>The brain lead content of test groups was higher than that of the control group (P < 0.01), and the lead content of the postnatal day 50 was higher than that in postnatal day 8 (P < 0.01). Compared with the control group, ASIC1a mRNA expression of 1.0% lead exposure in the hippocampus was uptrend (P < 0.01), ASIC1a protein expression of each test group was downtrend (P < 0.05), while for ASIC2a and ASIC2b mRNA and protein, there was no significant differences observed (P > 0.05).</p><p><b>CONCLUSION</b>ASIC1a expression in hippocampus can be changed by chronic lead exposure.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Efeitos Tardios da Exposição Pré-Natal
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RNA Mensageiro
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Canais de Sódio
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Ratos Wistar
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Toxicidade
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Canais Iônicos Sensíveis a Ácido
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Genética
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Hipocampo
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Chumbo
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Metabolismo
Limite:
Animals
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Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2008
Tipo de documento:
Article