Effects of domoic acid on membrane function of primary cultured rat glial cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 725-728, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-347230
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of domoic acid (DA) on membrane function of primary cultured rat glial cell.</p><p><b>METHODS</b>After the glial cells were treated with 6.4 x 10(-2), 6.4 x 10(-3) and 6.4 x 10(-4) micromol/L DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, the membrane fluidity and the permeability were measured to reflect the membrane function.</p><p><b>RESULTS</b>After treatment of DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase were inhibited significantly, the membrane fluidity decreased and the membrane permeability increased. The fluorescence polarization and microviscosity in the low, middle and high dosage treatment groups were 0.0626 +/- 0.0051, 0.0685 +/- 0.0097, 0.0648 +/- 0.0086 and 0.3154 +/- 0.0298, 0.3510 +/- 0.0571, 0.3286 +/- 0.0504 respectively, compared with the control group (0.0481 +/- 0.0069 and 0.2338 +/- 0.0372) (P < 0.01).</p><p><b>CONCLUSION</b>DA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Membrana Celular
/
Permeabilidade da Membrana Celular
/
Células Cultivadas
/
Neuroglia
/
Ratos Sprague-Dawley
/
Ácido Caínico
/
Fluidez de Membrana
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2008
Tipo de documento:
Artigo
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