Silicon dioxide-upregulated plasminogen activators inhibitor-1 protein expression is dependent on activation of extracellular signal-regulated kinase/activator protein-1 signaling pathway / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 387-390, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-347281
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of extracellular signal-regulated kinase (ERK)/activator protein-1 (AP-1) signaling pathway in SiO(2)-induced plasminogen activators inhibitor-1 (PAI-1) protein expression in human lung epithelial cells A549.</p><p><b>METHODS</b>A549 cells were cultured and then stimulated with 200 microg/ml SiO(2) for 0 approximately 24 h. To prevent AP-1 activity, Curcumin was added into culture medium before incubating with SiO(2) and transient TAM-67 transfection was performed. In addition, PD98059 was pretreated with cells to prevent ERK activity. The PAI-1 protein expression and ERK activity were evaluated by Western blot. The AP-1 DNA binding activity was tested by EMSA.</p><p><b>RESULTS</b>(1) At 4, 8 and 16 h after exposure to SiO(2), the fold change of AP-1 DNA binding activity (relative to the control group) were 1.3, 1.3, and 2.1, respectively (P < 0.05). 10, 25, 50 micromol/L Curcumin inhibited SiO(2)-induced PAI-1 protein expression (inhibition ratio 20%, 63%, 65%; P < 0.05). TAM-67 downregulated SiO(2)-induced PAI-1 protein expression (inhibition ratio 59%, P < 0.05). (2) SiO(2) activated ERK and PD98059 downregulated SiO(2)-induced PAI-1 protein expression (inhibition ratio 51%, P < 0.05). (3) PD98059 downregulated SiO(2)-induced AP-1 DNA binding activity (inhibition ratio 73%, P < 0.05).</p><p><b>CONCLUSION</b>ERK/AP-1 signaling pathway is responsible for SiO(2)-induced PAI-1 protein expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transdução de Sinais
/
Linhagem Celular
/
Inibidor 1 de Ativador de Plasminogênio
/
Dióxido de Silício
/
Fator de Transcrição AP-1
/
Biologia Celular
/
MAP Quinases Reguladas por Sinal Extracelular
/
Células Epiteliais
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Toxicidade
/
Pulmão
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2008
Tipo de documento:
Artigo
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