Intranasal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in gnotobiotic rats / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 452-455, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-347328
ABSTRACT
<p><b>OBJECTIVES</b>To assess the efficacy of plasmid DNA encoding pac gene of Streptococcus mutans (S. mutans) intranasally immunized in gnotobiotic rats and to compare the effect of two different delivery systems.</p><p><b>METHODS</b>Sprague Dawley rats, infected with S. mutans at 20 days of age, were intranasally immunized with plasmid pCIA-P (group A), Dosper-DNA complex (group B), Bupivacaine-DNA complex (group C). Control rats were either immunized with plasmid pCI (group D), distilled water (group E) or immunized intramuscularly (group F). All the rats were boosted 2 weeks later. ELISA determined the antibodies against the vaccines. Keyes caries score was used to evaluate the anti- caries effectiveness of the vaccines at the terminal study.</p><p><b>RESULTS</b>As for the antibody reactions, there were significantly (P < 0.01) differences between rats immunized with DNA vaccine and non-immunized rats. And rats in group B and C had the significantly (P < 0.01) higher level of specific salivary anti-PAc IgA antibodies and rats (group B, C, F) had the significantly (P < 0.01) higher specific serum anti-PAc IgG responses to DNA vaccine. Keyes scores of rats (group B and C) were significantly (P < 0.01) lower than others.</p><p><b>CONCLUSIONS</b>Intranasal immunization with plasmid pCIA-P encoding pac gene successfully reduces the caries and appears to be a promising approach against dental caries. Cationic liposome Dosper and local anesthetic bupivacaine could enhance the efficacy of DNA vaccine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Streptococcus mutans
/
Proteínas de Bactérias
/
Sangue
/
Imunoglobulina A
/
Imunoglobulina G
/
Administração Intranasal
/
Glicoproteínas de Membrana
/
Imunização
/
Resultado do Tratamento
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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