Effect of human serum albumin on cell attachment of human gingival epithelial cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 197-199, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-347396
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of human serum albumin (HSA) on cell attachment of human gingival epithelial cells (HGE).</p><p><b>METHODS</b>HGE were primary cultured with keratinocyte serum-free medium (KSFM) and dispase. The cultured cells were immunohistochemically stained by monoclonal anti-pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed.</p><p><b>RESULTS</b>The results showed significant decrease in cell numbers within 8 hours after HGE were inoculated, in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference.</p><p><b>CONCLUSIONS</b>HSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Poliestirenos
/
Albumina Sérica
/
Adesão Celular
/
Contagem de Células
/
Divisão Celular
/
Células Cultivadas
/
Biologia Celular
/
Células Epiteliais
/
Gengiva
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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