Hepatitis B x protein activated vascular endothelial growth factor expression through hypoxia inducible factor-1 pathway / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 433-436, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-347409
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether hepatitis B x protein (HBx) stimulates vascular endothelial growth factor (VEGF) through hypoxia inducible factor-1 (HIF-1 alpha) pathway.</p><p><b>METHODS</b>Two plasmids including pIRES-EGFP-HBx and pTK-Hyg were co-transfected to a hepatocellular carcinoma cell line SMMC-7721. With fluorescence-positive and fluorescence-negative hygromycin-resistant colonies selected, expressions of VEGF and HIF-1 alpha in protein or/and mRNA level were detected.</p><p><b>RESULTS</b>Fluorescence-positive cells were stably integrated with HBx, in which expression of HIF-1 alpha and VEGF were upregulated. Fluorescence-negative cells did not express HBx, VEGF or HIF-1 alpha.</p><p><b>CONCLUSION</b>HBx can activate VEGF through HIF-1 alpha pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Fatores de Transcrição
/
Transativadores
/
Regulação da Expressão Gênica
/
Linhagem Celular Tumoral
/
Fator A de Crescimento do Endotélio Vascular
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2003
Tipo de documento:
Artigo
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