Interleukin-27 affects IFN-gamma and IL-4 activities in children with asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 521-523, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-347554
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of interleukin (IL)-27 on INF and IL-4 activities in children with asthma.</p><p><b>METHODS</b>The levels of IFN-gamma and IL-4 in culture supernatants of peripheral blood mononuclear cells (PBMCs) from 23 asthmatic children were measured using ELISA. PBMCs were cultured with recombinant IL-27 (5 ng/mL or 0.5 ng/mL) for 12 hrs in vitro.</p><p><b>RESULTS</b>IFN-gamma levels in both 5 ng/mL and 0.5 ng/mL IL-27 treatment groups (85.9+/-12.2 and 8.9+/-2.3 microg/L, respectively) increased compared with those in the cytokine stimulated control group (P<0.01, P<0.05 respectively). The group treated with 5 ng/mL IL-27 had decreased significantly IL-4 levels compared with the cytokine stimulated control group (15.0+/-1.9 microg/L vs 77.0+/-15.6 microg/L; P<0.01).</p><p><b>CONCLUSIONS</b>IL-27 may be involved in the pathogenesis of asthma. It may affect Th1/Th2 cell activities and might be a new option for the treatment of asthma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Asma
/
Proteínas Recombinantes
/
Leucócitos Mononucleares
/
Células Cultivadas
/
Interleucina-4
/
Interferon gama
/
Interleucina-17
/
Alergia e Imunologia
Limite:
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
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