Analysis of HER2 gene amplification and its protein expression in 165 cases of breast carcinoma: comparison of chromogenic in-situ hybridization and immunohistochemistry / 中华病理学杂志
Chinese Journal of Pathology
; (12): 457-460, 2007.
Article
em Zh
| WPRIM
| ID: wpr-347759
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the sensitivity and specificity of chromogenic in-situ hybridization (CISH) in detecting HER2 gene amplification in breast carcinomas.</p><p><b>METHODS</b>HER2 oncogene amplification and its protein expression in 165 cases of breast carcinoma were investigated by immunohistochemistry (IHC) and CISH.</p><p><b>RESULTS</b>(1) CISH did not detect HER2 gene amplification in 107 cases of IHC negative tumors and 24 cases of IHC 1+ tumors. (2) CISH identified high copy numbers of HER2 gene amplification in 21/22 (95.5%) cases with IHC 3+. (3) In 12 HIC 2+ cases, CISH identified 3 cases of high copy number amplification, 6 cases of low copy number amplification and 3 cases without amplification.</p><p><b>CONCLUSIONS</b>HER2 gene amplification detection by CISH is highly sensitive and has a high concordance with IHC detection of the protein expression. It is concluded that CISH is a tool to evaluate HER2 gene status in breast cancer and can be an implement in conventional pathology laboratories.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Neoplasias da Mama
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Imuno-Histoquímica
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Amplificação de Genes
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Compostos Cromogênicos
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Hibridização In Situ
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Carcinoma Lobular
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Carcinoma Ductal de Mama
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Carcinoma Intraductal não Infiltrante
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Receptor ErbB-2
Limite:
Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2007
Tipo de documento:
Article