Analysis of the methylation in the promoter of LRP15 gene and its expression / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 188-191, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-347799
ABSTRACT
To study the methylation in the promoter of LRP15 gene and its relationship with gene expression and to explore the possible mechanism of regulating LRP15 gene methylation, the methylation in the promoter of LRP15 gene in K562 cell line was detected by MS-PCR. Then K562 was exposed to 5-aza-2'-deoxycytidine (CdR) and trichostatin (TSA), to determine whether the silencing of LRP15 gene by de novo methylation could be reversed. As a result, it was confirmed by MS-PCR that the promoter of LRP15 was hypermathylated in K562 cell line, and lost its transcription activity. After CdR, with or without TSA, the silencing of LRP15 gene by de novo methylation can be reversed. Observation demonstrated that the expression of LRP15 was controlled by methylation in its promoter in K562. It is suggested that methyltransferase inhibitor and deacetylase inhibitor may be effective agents in leukemia therapy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Azacitidina
/
Metilases de Modificação do DNA
/
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Regiões Promotoras Genéticas
/
Metilação de DNA
/
Células K562
/
Inibidores Enzimáticos
/
Inibidores de Histona Desacetilases
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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