MAPK signal pathway plays a role in proliferation of Jurkat cells induced by ouabain / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 126-129, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-347812
ABSTRACT
The object was to study the effect of ouabain on Jurkat cells and its possible mechanism. The effect of ouabain of low concentration on Jurkat cells was confirmed by MTT, while c-myc gene transcription was measured by RT-PCR, and the phosphorylation of MAPK (ERK1/2) as well as the expression of c-myc gene was tested by Western blot respectively. The results showed that ouabain at low concentration could induce the proliferation of Jurkat in a time-and dose-dependent manner. At the same time, the phosphorylation of MAPK (ERK1/2) and the expression of c-myc gene was enhanced. In conclusion, ouabain stimulates the intracellular MAPK signal pathway by acting on the Na, K-ATPase, and thus induce the proliferation of Jurkat cells, in which the regulation of c-myc gene expression may be involved.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ouabaína
/
Farmacologia
/
Fosforilação
/
Fisiologia
/
Fatores de Tempo
/
RNA Mensageiro
/
Western Blotting
/
Proteínas Proto-Oncogênicas c-myc
/
Células Jurkat
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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