Identification bcr-abl fusion gene in leukemia cells with oligonucleotide microarray / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 743-747, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-347871
ABSTRACT
To explore the application value of bcr-abl fusion gene deterction microarray in diagnosis, typing, choosing of treatment variant and prognosis judgment, probe for fusion gene detection was designed, oligonucleotide microarray was prepared; total RNA was extracted, reverse-transcripted and labeled by fluorescence, then cDNA was hybridized with microarray in order to detect bcr-abl fusion gene in leukemia cells. The results showed that better reaction conditions were gained by exploration of hybridizotion temperature and elution conditions, bcr-abl fusion gene in leukemia cells was detected by prepared miccroarray. In conclusion, oligonucleotide microarray is effective in detecting the fusion gene and has some unique advantages and certain clinical application value, but has some deficiency too. If microarray can be improved further, it could be used widely in the field of hematology.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Leucemia
/
Regulação Leucêmica da Expressão Gênica
/
Reprodutibilidade dos Testes
/
Proteínas de Fusão bcr-abl
/
Células HL-60
/
Células K562
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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