Nasal immunization with co-expression plasmid harboring genes encoding porphyromonas gingivalis FimA and human interleukin-15 in mice / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 177-179, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-348070
ABSTRACT
<p><b>OBJECTIVE</b>To observe the antibody responses induced by recombinant plasmid plRES-fimAIL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA.</p><p><b>METHODS</b>BABL/c mice were immunized with recombinant plasmids pIRES-fimAIL15 and pIRES-fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA.</p><p><b>RESULTS</b>Nasal immunization with plasmids pIRESfimAIL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES -fimAIL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA (P<0.05).</p><p><b>CONCLUSION</b>Nasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL-15 has a positive regulation effect to sIgA response.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Imunização
/
Porphyromonas gingivalis
/
Interleucina-15
/
Camundongos Endogâmicos BALB C
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
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