Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 738-741, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-348196
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.</p><p><b>METHODS</b>A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.</p><p><b>RESULTS</b>When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.</p><p><b>CONCLUSION</b>Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Pancreáticas
/
Patologia
/
RNA Mensageiro
/
Transfecção
/
Regulação para Baixo
/
Regulação Neoplásica da Expressão Gênica
/
Ciclo Celular
/
Apoptose
/
Anexina A1
/
RNA Interferente Pequeno
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2007
Tipo de documento:
Artigo
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