Primary analysis on protein maps of Helicobacter pylori strains associated with gastric carcinoma / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.</p><p><b>METHODS</b>The whole-cell proteins of H. pylori were separated by two-dimensional electrophoresis (2-DE). The protein maps of four H. pylori strains associated with gastric carcinoma and nine strains that isolated from patients with non-gastric carcinoma were then compared by ImageMaster 2D v3.1. MALDI-TOF mass spectrometry was performed to identify the proteins of interest. The proteins were searched by software mascot and identified by peptide fingerprint map.</p><p><b>RESULTS</b>Three proteins seemed to be associated with gastric carcinoma including acylneuraminate cytidylyltransferase with Mowse score 79 with the sequence coverage of 32%. The other two had no unambiguous protein to match.</p><p><b>CONCLUSION</b>Acylneuraminate cytidylyltransferase seemed to be a specific H. pylori protein associated with the presence of gastric carcinoma. Other two were novel proteins that might be associated with gastric carcinoma. However, the mechanism needs to be explored.</p>