Isolation, identification and sequence analyses of dengue virus type 2 strain GD19/2001 / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 288-290, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-348847
ABSTRACT
<p><b>OBJECTIVE</b>To identify the virus isolated from Jiangmen, Guangdong province and to discuss the possible origin.</p><p><b>METHODS</b>Using characteristics of indirect fluorescent antibody tests (IFA), reverse transcription-polymerase chain reaction (RT-PCR), mouse neurovirulence and cell culture to identify the isolated virus. According to the nature of dengue virus type 2 NGC strain, two pairs of primers were designed. The structural protein gene of isolated dengue virus type 2 strain was then amplified by RT-PCR, cloned into pMD18-T vector and sequenced.</p><p><b>RESULTS</b>Twenty-two of 37 serum samples showed a positive reaction to dengue antibody IgG, and 36 of 37 with IgM with the highest antibody titer 1640. Ten samples were resulted in a cytopathy on C6/36 cells and showed a neurovirulence in suckling mice when inoculated intracerebrally. The structural gene of new isolate GD19/2001 containing 2 325 nucleotides which encoded 774 amino acids. Data on nucleotide homology were 98%, 96%, 94%, 94%, 92%, 92%, 92% and 91% compared with TSV01, GD06/93, NGC and 44, ThNH81/93, 04 and GD08/98, and S1 respectively.</p><p><b>CONCLUSION</b>The isolated virus from Jiangmen, Guangdong province belonged to dengue virus type 2, which might come from Australia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Sangue
/
DNA Viral
/
RNA Viral
/
China
/
Reação em Cadeia da Polimerase
/
Epidemiologia
/
Imunofluorescência
/
Análise de Sequência de DNA
/
Dengue
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2003
Tipo de documento:
Artigo
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