Cytogenetic and molecular characterization of partial trisomy 4q and partial monosomy 10q in a patient / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 153-157, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-349022
ABSTRACT
<p><b>OBJECTIVE</b>To ascertain the karyotype of a girl with moderate mental retardation and growth retardation, perform correlation analysis between chromosomal variation and phenotype, and investigate the application and superiority of array-based comparative genomic hybridization (array-CGH) in clinical cytogenetic diagnosis.</p><p><b>METHODS</b>G-banded chromosome analysis, array-CGH, fluorescence in situ hybridization (FISH) and real-time quantitative PCR (RQ-PCR) were used to ascertain the karyotype of the patient and her relatives.</p><p><b>RESULTS</b>G-banding analysis of the patient showed a derivative chromosome 10 with an extra fragment on its long arm terminal, both her father and grandmother had an apparently balanced translocation t(4;10)(q25;q26). Array-CGH revealed that the breakpoint on chromosome 4 was located at 4q26. In addition, a microdeletion of about 0.54 Mb del(10)(q26.3) was identified from the patient. FISH and RQ-PCR confirmed that the del(10)(q26.3) was also present in both her father and grandmother.</p><p><b>CONCLUSION</b>No recognizable phenotype was associated with del(10)(q26.3). The abnormal phenotypes presented in the patient may be ascribed to the 4q26-q35.2 triplication. Further more, compared with conventional cytogenetic analysis, array-CGH is of high resolution and high accuracy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Trissomia
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Cromossomos Humanos Par 4
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Cromossomos Humanos Par 10
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Reação em Cadeia da Polimerase
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Deleção Cromossômica
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Hibridização in Situ Fluorescente
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Análise Citogenética
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Hibridização Genômica Comparativa
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Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2010
Tipo de documento:
Artigo
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