Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell / 浙江大学学报·医学版

ABSTRACT

OBJECTIVE: To develop a rapid screening method for xenoestrogens and to screen the estrogenicity of some environmental chemicals. METHODS: The E-SCREEN test was developed based on proliferation of human breast cancer cells (MCF-7), and the estrogenicity of diethylstilbestrol, 4-hydrotamoxifen and endosulfan was assessed. RESULTS: The E-SCREEN detected estradiol at very low concentration as 1x10(-13) mol/L. It was found that diethylstilbestrol was a full agonist of estrogen receptor, endosulfan was a partial agonist, while 4 hydrotamoxifen lacked estrogenic effects at this assay. CONCLUSION: The E-SCREEN test is sensitive, rapid, easy to perform and, therefore, suitable for large scale screening for estrogenicity of environmental chemicals.