Influence of different DNA extractions on the identification of streptococcus sanguis group by arbitrary primed polymerase chain reaction / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 464-466, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-349418
ABSTRACT
OBJECTIVE:
To assess the influence of different DNA extractions on the identification of streptococcus sanguis group (SSG) species by arbitrary primed polymerase chain reaction (AP-PCR).METHODS:
AP-PCR was used to distinguish SSG species by designing 25bp arbitrary primer 5'AAG AGA GGA GCT AGC TCT TCT TGG A 3', and the genomic DNA was extracted by 3 methods.RESULTS:
There were great differences in the main band of DNA polymorphism among SSG species. The similar band could be got from the different DNA extractions in the same species.CONCLUSION:
Different DNA extractions have no influence on the identification of SSG.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2002
Tipo de documento:
Artigo
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