Identification of a novel HLA allele, HLA-DRB1*03:80, by sequencing-based typing / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 509-512, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-349681
ABSTRACT
This study was aimed to identify a novel HLA-DRB1 allele from a Chinese potential hemopoietic stem cell donor of Northeast China. A rare HLA-DRB1 allele was initially detected by Luminex PCR-SSO typing, then the sample was sequenced by sequence-based typing (SBT) and the alignments of sample's alleles was identified by single allele-specific sequencing strategy. The results revealed the existence of a new allele which differs from the closest matching allele DRB1*0306 by a single nucleotide substitution at position 239, where C→G in exon 2, resulting in an amino acid exchange from Thr to Arg at codon 51. It is concluded that a novel allele has been confirmed and its name DRB1*0380 is officially assigned by the WHO Nomenclature Committee in February 2012.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Análise de Sequência de DNA
/
Povo Asiático
/
Alelos
/
Cadeias HLA-DRB1
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2014
Tipo de documento:
Artigo
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