Virulent gene prevalence of foodborne Listeria monocytogenes in China in 2005 / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 1097-1101, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-349915
ABSTRACT
<p><b>OBJECTIVE</b>To study the virulent gene prevalence of foodborne Listeria monocytogenes (LM) isolated from China.</p><p><b>METHODS</b>78 LM isolates derived from raw meat, cooked food, aquatic products and vegetables of 13 provinces and cities.LM isolates were investigated for prevalence of virulence genes (LIPI-1 (prfA, plcA, hly, mpl, actA, plcB); LIPI-2 (inlA, inlB), and iap) by PCR method.</p><p><b>RESULTS</b>87.2% (68/78) of the isolates were prfA positive, 98.7% (77/78) of the isolates were plcA, actA and plcB positive, 97.4% (76/78) of the isolates were hly positive, 87.2% (68/78) of the isolates were mpl positive, 92.3% (72/78) of the isolates were inlA positive, 100% (78/78) of the isolates were inlB positive, 98.7% (77/78) of the isolates were iap positive. Among 21 virulent gene negative isolates, there was 7 isolates lack of two or more virulence genes. The rate of virulence genes deletion isolates from cooked meat was 31.3% (10/32), the rate of virulence genes deletion isolates from raw meat was 16.1% (5/31), the rate of virulence genes deletion isolates from vegetables was 36.4% (4/11) and rate of virulence genes deletion isolates from seafood was 50% (2/4). No significant difference was found (χ(2) = 3.721, P > 0.05). The virulence gene array-1 strains were dominant among these isolates.</p><p><b>CONCLUSION</b>Among 78 LM isolates, prevalent of virulent genes were different except inlB, virulence genes of LIP-1 were deleted prevalently among isolates, virulence gene deletion patterns were diverse.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virulência
/
Contaminação de Alimentos
/
China
/
Epidemiologia
/
Fatores de Virulência
/
Microbiologia de Alimentos
/
Doenças Transmitidas por Alimentos
/
Genética
/
Listeriose
/
Listeria monocytogenes
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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