Clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 736-741, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-350026
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue (MALT) lymphoma.</p><p><b>METHODS</b>Six cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene.</p><p><b>RESULTS</b>In the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases.</p><p><b>CONCLUSION</b>The tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Sequência de Bases
/
Rearranjo Gênico de Cadeia Pesada de Linfócito B
/
Proteínas de Fusão Oncogênica
/
Núcleo Celular
/
Transformação Celular Neoplásica
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
/
Linfoma de Zona Marginal Tipo Células B
/
Citoplasma
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2007
Tipo de documento:
Artigo
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