Study on osteogenic ability of chitosan/beta-tricalcium phosphate scaffold combined with human bone morphogenetic protein / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 464-467, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-350309
ABSTRACT
<p><b>OBJECTIVE</b>Using chitosan (CS)/beta-tricalcium phosphate (TCP)/recombinant human bone morphogenetic protein (rhBMP)-2 for the reconstruction of rabbits' mandible defect, to prove the feasibility of CS/beta-TCP as an injectable bone tissue engineering scaffold material.</p><p><b>METHODS</b>Twenty-four New Zealand white rabbits were randomized into 4 groups on average Experimental group 1 embedding CS/beta-TCP/rhBMP-2, experimental group 2 embedding CS/ beta-TCP, control group 1 embedding autograft bone group, control group 2 embedding nothing. At 2, 4 and 8 weeks after surgery, all rabbits were executed group by group. The new bone growth situations were observed with hematoxylin-eosin staining and immunofluorescence microscopy, the bone mineral density was detected by bone sonometers.</p><p><b>RESULTS</b>After 2, 4, 8 weeks, there was significant difference among the areas of bone regeneration of all groups. The effect of experimental group 1 was better than experimental group 2. There was significant difference at different times, the areas of bone regeneration was gradually increased with time. The area of stained yellow in experimental group 1 was larger, the area of stained red was smaller. The quantities of bone density in experimental group 1 at every time after surgery were significantly higher than experimental group 1 and control group 2, but had no statistical significance with control group 1.</p><p><b>CONCLUSION</b>CS/beta-TCP/rhBMP-2 has good biocompatibility, degradability and the capacity of guided and inducing osteogenesis. CS/beta-TCP as a good injection of carrier could become a promising carrier for rhBMP-2 and potential new degradable biological material for repairing bone defect in clinical application.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Osso e Ossos
/
Regeneração Óssea
/
Proteínas Recombinantes
/
Fosfatos de Cálcio
/
Fator de Crescimento Transformador beta
/
Transplante Ósseo
/
Proteínas Morfogenéticas Ósseas
/
Engenharia Tecidual
/
Quitosana
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2010
Tipo de documento:
Artigo
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