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Effects of indium on micronucleus formation in human peripheral blood lymphocytes / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 563-565, 2015.
Artigo em Chinês | WPRIM | ID: wpr-350538
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cytotoxicity of indium chloride (InCl₃) and its effects on micro-nucleus formation in primary human lymphocytes cultured in vitro.</p><p><b>METHODS</b>The CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl₃(4, 40, 80, 200, 500, and 1 000 µmol/L) in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InCl₃and the effects of anti-oxidant vitamin C on micronucleus frequency.</p><p><b>RESULTS</b>Lymphocytes exposed to InCl₃of no less than 500 µmol/L had significantly lower survival rates than those in the control group (P < 0.05). Lymphocytes exposed to 80 µmol/L InCl₃had a significantly higher micronucleus frequency than those in the control group (P < 0.05). However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200 µmol/L InCl₃. Lymphocytes cultured in whole blood and exposed to 500 or 1000 µmol/L InCl₃had a significantly increased micronucleus frequency than those in the control group (P < 0.001). The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 µmol/L vitamin C.</p><p><b>CONCLUSION</b>InCl₃can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Técnicas In Vitro / Dano ao DNA / Linfócitos / Núcleo Celular / Estresse Oxidativo / Citocinese / Toxicidade / Índio / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Técnicas In Vitro / Dano ao DNA / Linfócitos / Núcleo Celular / Estresse Oxidativo / Citocinese / Toxicidade / Índio / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2015 Tipo de documento: Artigo