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Effect of silicon dioxide nanoparticles on expression and DNA methylation of PARP-1 gene in HaCaT cells / 中华劳动卫生职业病杂志
Article em Zh | WPRIM | ID: wpr-350561
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of silicon dioxide nanoparticles on the expression and promoter region CpG islands methylation of (Poly [ADP-ribose] polymerase 1, PARP-1) gene in human HaCaT Cell.</p><p><b>METHODS</b>HaCaT Cells were treated with nm-SiO₂at 0, 2.5, 5 and 10 µg/mL and micro-SiO₂at 10 µg/ml for 24 h and DAC treatment was given at 10 µg/ml group for 48 h. Real-time PCR and western blot assay was used to detect the expression of PARP-1 mRNA and protein. BSP (Bisulfite Pyrosequence, BSP) assay was used to detect the promoter region CpG islands methylation status of PARP-1 gene.</p><p><b>RESULTS</b>After exposure to nano-SiO₂particles, compared to CTRL group, the mRNA and protein expression of PARP-1 in micro-SiO₂and 2.5 µg/ml group unchanged, but he mRNA and protein expression of PARP-1 in 5, 10 µg/ml as well as DAC group was down-regulated and there are statistical significance between CTRL group and 5, 10 µg/ml as well as DAC group and the PARP-1 promoter region CpG islands showed methylation.</p><p><b>CONCLUSION</b>nano-SiO₂can down-regulate PARP-1 expression in HaCaT Cell and this is associated with the change in the methylation of PARP-1 gene promoter region CpG islands induced by nano-SiO₂particles.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: RNA Mensageiro / Regiões Promotoras Genéticas / Poli(ADP-Ribose) Polimerases / Dióxido de Silício / Ilhas de CpG / Metilação de DNA / Linhagem Celular Tumoral / Nanopartículas / Poli(ADP-Ribose) Polimerase-1 / Metabolismo Limite: Humans Idioma: Zh Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2015 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: RNA Mensageiro / Regiões Promotoras Genéticas / Poli(ADP-Ribose) Polimerases / Dióxido de Silício / Ilhas de CpG / Metilação de DNA / Linhagem Celular Tumoral / Nanopartículas / Poli(ADP-Ribose) Polimerase-1 / Metabolismo Limite: Humans Idioma: Zh Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Ano de publicação: 2015 Tipo de documento: Article