Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 947-950, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-350684
ABSTRACT
<p><b>OBJECTIVE</b>To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.</p><p><b>METHOD</b>We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.</p><p><b>RESULT</b>Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.</p><p><b>CONCLUSION</b>Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Padrões de Referência
/
Tartarugas
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Classificação
/
Análise de Sequência de DNA
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Proteínas de Répteis
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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