DNA barcoding application of mitochondrial COI gene sequence in medicinal fish of Culter (Pisces: Cyprinidae) / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 943-946, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-350685
ABSTRACT
The sequence variation of medicinal fish of Culter (Pisces Cyprinidae) was analyzed by using cytochrome c oxidase subunit I (COI) sequencing collected from different regions of the Yangtze River basin, and we examine whether barcoding of COI can be used to discriminate medicinal fish of Culter. The AT content in the COI region of medicinal fish of Culter was higher than that of GC, which was similar with other species of Cypriniformes. Ninty-six percent of nucleotide changes were observed at the 3rd codon position of COI sequence, but the amino acid compositions translated by COI sequences of all Culter fish stayed the same. It is suggested that most synonymous mutations might occur at the 3rd position. The average Kimura-2-parameter (K2P) distance within-species was lower than 1%, and the K2P distance of pairwise-species was 10 times as much as that of within-species. The phylogenetic tree estimated by Neighbour-joining method indicated that species within genera invariably clustered, and generally so did individuals within species. Individuals from operational taxonomic units designated as different Culter species, supporting morphological evidence for each of these being separate species. It is suggested that the COI barcoding can be used to identify medicinal fish species of Culter.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
DNA Mitocondrial
/
Cyprinidae
/
Dados de Sequência Molecular
/
China
/
Classificação
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Proteínas de Peixes
/
Código de Barras de DNA Taxonômico
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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