Peptides analysis in digested edible bird's nest by HPLC-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 714-719, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-350699
ABSTRACT
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Espectrometria de Massas
/
Aves
/
Glicoproteínas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Sequência de Aminoácidos
/
Proteólise
/
Medicina Tradicional Chinesa
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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