Effects of Rehmannia glutinosa oligosaccharides on proliferation of 3T3-L1 adipocytes and insulin resistance / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 403-407, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-350928
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of 3T3-L1 adipocytes and insulin resistance.</p><p><b>METHOD</b>3T3-L1 preadipocytes were cultured, the proliferation of 3T3-L1 preadipocytes was detected by MTT method. Insulin resistant 3T3-L1 adipocytes cell model was induced by dexamethasone and the change of glucose concentration in cell culture was determined after ROS treatment.</p><p><b>RESULT</b>In the high glucose DMEM culture media, MTT method showed that the absorbance at 570nm of 3T3-L1 preadipocytes was increased and that of 3T3-L1 adipocytes was decreased. ROS significantly increased glucose consumption in 3T3-L1 preadipocytes and adipocytes culture in a concentration-dependent manner. ROS improved the sensitivity of 3T3-L1 adipocytes to insulin.</p><p><b>CONCLUSION</b>ROS can promote the proliferation of 3T3-L1 preadipocytes, inhibite the proliferation of 3T3-L1 adipocytes, and also, significantly improve insulin resistance induced by dexamethasone.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligossacarídeos
/
Farmacologia
/
Plantas Medicinais
/
Resistência à Insulina
/
Dexametasona
/
Química
/
Adipócitos
/
Biologia Celular
/
Rehmannia
/
Células 3T3-L1
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Artigo
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