Determination of epicatechin in Rhizoma Fagopyri dibotoryis by HPLC-ECD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a quantitative method of determination of epicatechin in Rhizoma fagopyri dibotoryis by HPLC-electrochemical detection.</p><p><b>METHOD</b>The sample was separated on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol-0.1 mol x L(-1) phosphate buffer (13, pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the applied potential was + 600 mV.</p><p><b>RESULT</b>The linear range of epicatechin was 0.004 875-1.56 microg, r = 0.999 9. The average recovery was 100.7% with RSD 1.9% (n = 5). The content of epicatechin was different in nine samples purchased.</p><p><b>CONCLUSION</b>The method is good reproducible and can be used to determine epicatechin in Rhizoma fagopyri dibotoryis.</p>