Effects of salvia miltiorrhiza injection on gentamicin-induced expression of nitric oxide synthase isoforms in guinea pig cochlea / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 246-249, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-351186
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of salvia miltiorrhiza injection (SM) on gentamicin (GM)-induced expression of nitric oxide synthase (NOS) isoforms in guinea pig cochlea, and to explore the protective mechanism of SM on GM-induced ototoxicity.</p><p><b>METHODS</b>40 guinea pigs were randomly assigned to 4 groups control group, GM group, SM group and GM plus SM group. Expression of NOS isoforms in the guinea pig cochlea was detected by the SABC method of immunohistochemistry and microscope image analysis technique. Auditory threshold was tested by auditory brainstem response (ABR) measurement.</p><p><b>RESULTS</b>Inducible NOS (iNOS/NOS II) expression and ABR threshold in GM plus SM group were both significantly declined as compared with those in GM group (P < 0.01). Moreover, change of iNOS expression was in high correlation with that of ABR threshold ([r] > 0.7, P < 0.01). While expression of neuronal NOS (nNOS/NOS I) and endothelial NOS (eNOS / NOS III) showed no significant differences in all groups.</p><p><b>CONCLUSION</b>SM had no effect on the expression of nNOS and eNOS, but could inhibit iNOS high-expression induced by GM to reduce excessive generation of NO, therefore SM could protect against GM ototoxicity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Gentamicinas
/
Regulação para Baixo
/
Química
/
Cóclea
/
Substâncias Protetoras
/
Salvia miltiorrhiza
/
Óxido Nítrico Sintase Tipo I
/
Óxido Nítrico Sintase Tipo II
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2011
Tipo de documento:
Artigo
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