Separation and authentication of tilianin and quality standards of semen of Dracocephalum moldavia / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1845-1849, 2015.
Article
em Zh
| WPRIM
| ID: wpr-351252
Biblioteca responsável:
WPRO
ABSTRACT
Tilianin was separated and authenticated from the seeds of Dracocephalum moldavia, a Uygur medicine, by chromatographic technique and spectroscopic method. The purity of tilianin is more than 98% determined by HPLC area normalization method. Thin layer chromatography (TLC) method was used to separate tilianin from D. moldavia by mixture of chloroform-methanol (5: 1) as a developing solvent on high performance silicagel precoated plate (SGF254) and using aluminium trichloride as a chromogenic agent for qualitative identification of D. moldavia. To establish a HPLC method for quantitative analysis of D. moldavia, tilianin was used as a Quantitative marker and separated on a C18 (4.6 mm x 250 mm, 5 μm) column with acetonitrile-01% formic acid (25: 75) as the mobile phase and detected at 330 nm. The calibration curve of tilianin displayed ideal linearity over the range of 0.617 2-123.44 μg x mL(-1) with a regression equation of Y = 33.773X - 0.824 8 (r = 1). The average recovery of tilianin was 101.0% with RSD of 3.7%. The RSD values of intra-day and inter-day precision were less than 2%. The content of tilianin in 4 batches of the authenticated semen of D. Moldavia was between 0.016 and 0.187 mg x g(-1). The qualitative and quantitative method established is suitable for the quality evaluation and assessment of semen of D. Moldavia.
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1
Índice:
WPRIM
Assunto principal:
Controle de Qualidade
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Padrões de Referência
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Flavonoides
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Medicamentos de Ervas Chinesas
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Espectroscopia de Ressonância Magnética
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Química
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Cromatografia Líquida de Alta Pressão
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Cromatografia em Camada Fina
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Lamiaceae
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Glicosídeos
Tipo de estudo:
Qualitative_research
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Article