A novel trichostatin analogue culture of Streptomyces sp. CPCC 203909 / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1751-1754, 2015.
Article
em Zh
| WPRIM
| ID: wpr-351271
Biblioteca responsável:
WPRO
ABSTRACT
By using a cell-based high throughput screening model for the CLA-1 up-regulator, Streptomyces 203909 was found to produce up-regulator of CLA-1. A novel trichostatin analogue was isolated from the rice fermentation of Streptomyces sp. CPCC 203909by a combination of various chromatographic techniques including column chromatography (CC) over silica gel, flash C18 CC, and reversed-phase HPLC. Its structure was identified as (-)-(R,2E,4Z)-7-[(4'-dimethylamino) phenyl]-4,6-dimethyl-7-oxohepta-2,4-dienoyl-L-glutamine (1) by the spectroscopic and chemical methods, and combination with the CD spectroscopy and Marfey's method. In the prelimi- nary assays, Compound 1 showed cytotoxicity against human embryonic kidney 293 cell line with IC50 value 35.3 [µmol · L(-1).
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Índice:
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Assunto principal:
Farmacologia
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Streptomyces
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Estrutura Molecular
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Sobrevivência Celular
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Química
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Células Hep G2
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Fermentação
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Ácidos Hidroxâmicos
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Article