Expression of porcine interleukin-18 in baculovirus/insect cells / 生物工程学报
Chinese Journal of Biotechnology
; (12): 118-123, 2011.
Article
em Zh
| WPRIM
| ID: wpr-351527
Biblioteca responsável:
WPRO
ABSTRACT
IL-18, as a polyphonic cytokine, is important in immune response and physiologic function. We designed one pair of primers, amplified the porcine IL-18 gene fused with a C-terminal 6xHistidine tag, and then subcloned into the pFastBacDual of Baculovirus transfer vector and transformed into DH10Bac containing a shuttle vector of Bacmid. After co-transfecting the recombinant plasmid into insect cells, the 18 kDa expressed protein of porcine IL-18 was detected by SDS-PAGE; the specificity of expressed protein was confirmed by Western blotting. The purified porcine IL-18 protein induced obvious proliferation of porcine T lymphocytes in vitro, which indicated that the expression of IL-18 had high biological activity.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Suínos
/
Proteínas Recombinantes
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Transfecção
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Células Cultivadas
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Baculoviridae
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Interleucina-18
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Alergia e Imunologia
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Vetores Genéticos
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Genética
/
Insetos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Article