Cyclosporin A Inhibits Albumin Synthesis in Huh7 Cells
The Korean Journal of Internal Medicine
; : 314-319, 2011.
Article
em En
| WPRIM
| ID: wpr-35157
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS: Hypoalbuminemia occurs frequently in renal transplant recipients immediately after renal transplantation. We studied the regulation of hepatic albumin synthesis by cyclosporin A (CsA) in Huh7 cells. METHODS: Huh7 cells were incubated with various concentrations of CsA for 4, 8, 16, and 24 hours. Albumin was measured in Huh7 cell-conditioned medium by sandwich enzyme-linked immunosorbent assay and Western blot. Albumin mRNA expression was analyzed by Northern blotting in CsA-treated cells. RESULTS: CsA (10(-7)-10(-4) M) inhibited albumin synthesis in Huh7 cells in a dose- dependent manner. A Western blot analysis for albumin in the conditioned medium released from CsA-treated (10(-7)-10(-5) M) cells also showed significant inhibition of albumin synthesis in a dose-dependent manner. Vehicle (olive oil) did not affect albumin synthesis. In contrast, a Northern blot analysis revealed no inhibition of albumin mRNA expression by CsA at any time point from 1-24 hours, indicating that the inhibition of albumin synthesis occurred at the translational level. CONCLUSIONS: Our results suggest that inhibition of hepatic albumin synthesis by high dose CsA contributes to the hypoalbuminemia in renal transplant recipients.
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Índice:
WPRIM
Assunto principal:
Fatores de Tempo
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RNA Mensageiro
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Albumina Sérica
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Ensaio de Imunoadsorção Enzimática
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Regulação Neoplásica da Expressão Gênica
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Sobrevivência Celular
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Western Blotting
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Northern Blotting
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Ciclosporina
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Meios de Cultivo Condicionados
Limite:
Humans
Idioma:
En
Revista:
The Korean Journal of Internal Medicine
Ano de publicação:
2011
Tipo de documento:
Article