Substrate specificity of carotenoid 3',4'-desaturase from Deinococcus radiodurans / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1451-1455, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-351574
ABSTRACT
To examine the substrate specificity of carotenoid 3',4'-desaturase (DR2250) from Deinococcus radiodurans, we amplified the dr2250 gene by using PCR methods. The PCR products were digested by Hind III-BamH I and ligated into the vector pUC19, yielding recombinant vector pUC-CRTD. We analyzed the carotenoids of E. coli transformants containing pACCRT-EBI(Eu) and (or) pRK-CRTC and (or) pUC-CRTD. Our results demonstrated that DR2250 had substrate specificity on the carotenoids with hydroxyl group at C1 (1').
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxirredutases
/
Especificidade por Substrato
/
Carotenoides
/
Deinococcus
/
Escherichia coli
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Artigo
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