Determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>An RP-HPLC procedure was established for the determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder.</p><p><b>METHOD</b>An RP-HPLC analytical procedure was developed using Hypersil ODS2 C18 column (4.6 mm x 250 mm, 5 microm) with methanol-0.5% glaceal acetic acid (4.595.5) as mobile phase, and a wavelength of 280 nm for UV detection.</p><p><b>RESULT</b>The linear range was 3.004-45.06 microg x m(L-1) (r = 0.999 5, n = 6) for danshensu, and 0.300-4.509 microg x mL(-1) (r = 0.999 3, n = 6) for protocatechuic aldehyde. The average recoveries were 99.1% and 97.9%, respectively.</p><p><b>CONCLUSION</b>The method was stable, accurate, and reproducible, and can be used for the quality control of Guanxinning injection powder.</p>