Determination of akebia saponin D in root of Dipacus asperoides by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 726-739, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-351773
ABSTRACT
<p><b>OBJECTIVE</b>To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.</p><p><b>METHOD</b>An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (3070) as mobile phase and the detection wavelength was 212 nm.</p><p><b>RESULT</b>The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.</p><p><b>CONCLUSION</b>This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Controle de Qualidade
/
Saponinas
/
Química
/
China
/
Cromatografia Líquida de Alta Pressão
/
Ecossistema
/
Raízes de Plantas
/
Dipsacaceae
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Artigo
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