Estrogenic activity and its mechanism of ethanol extract from black soybean / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 825-828, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-351782
ABSTRACT
<p><b>OBJECTIVE</b>To explore the estrogenic activity and its mechanism of ethanol extract from black soybean (BSE).</p><p><b>METHOD</b>The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of BSE. And the possible mechanisms were addressed using RT-PCR measurements in which pure estrogen receptor antagonist ICI182,780 was employed as a tool.</p><p><b>RESULT</b>The estrogenic activity of BSE at various concentrations (1-1000 microg x mL(-1)), expressed as proliferative effect (PE) relative to that of solvent control, was examined. The results indicated that, at low concentration range (10-200 microg x mL(-1)), BSE was able to induce MCF-7 cell growth with a maximum at 100 microg x mL(-1). The results of RT-PCR showed that pS2 and PR mRNA could be significantly induced by BSE in MCF-7 cells, which was relative to the concentration of BSE. Moreover, the specific estrogen receptor antagonist, ICI182,780 could block these reactions.</p><p><b>CONCLUSION</b>Ethanol extract of black soybean can stimulate the growth of MCF-7 cells and increase the expression of estrogen receptor-responsive gene, suggesting that the estrogenic effects of BSE are mediated by the estrogen receptor.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Plantas Medicinais
/
Sementes
/
Glycine max
/
Neoplasias da Mama
/
RNA Mensageiro
/
Medicamentos de Ervas Chinesas
/
Receptores de Progesterona
/
Química
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Artigo
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