Studies on plantlet regeneration and propagation of Gentiana stramines / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 797-800, 2006.
Article
em Zh
| WPRIM
| ID: wpr-351792
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.</p><p><b>METHOD</b>Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.</p><p><b>RESULT</b>The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.</p><p><b>CONCLUSION</b>Aseptic seeding of G. stramines can be quickly propagated by shoot culture.</p>
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Assunto principal:
Farmacologia
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Reguladores de Crescimento de Plantas
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Plantas Medicinais
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Regeneração
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Caules de Planta
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Meios de Cultura
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Gentiana
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Plântula
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Técnicas de Cultura de Tecidos
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Article