Purification of plasmin-alpha2-antiplasmin complex by lysine-sepharose 4B affinity chromatography employing sequential elutions / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 355-358, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-352066
ABSTRACT
To easily obtain plasmin-alpha(2)-antiplasmin complex (PAP) with high purity, the purification procedure was improved by authors. After urokinase activated fresh human platelet-deficient plasma had been applied to Lysine-Sepharose 4B affinity chromatography column, elutions were sequentially performed by several eluents with different ingredients. The results showed that 3.0 mg PAP could harvested from 100 ml fresh plasma by this method and the whole procedure could be finished within several hours. In conclusion, this procedure is simple, rapid, economical and high-yield.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sefarose
/
Western Blotting
/
Alfa 2-Antiplasmina
/
Cromatografia de Afinidade
/
Fibrinolisina
/
Eletroforese em Gel de Poliacrilamida
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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