Effect of arsenic trioxide on telomerase and telomerase reverse transcriptase in KM3 cell line / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 346-349, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-352068
ABSTRACT
To explore the effects of arsenic trioxide on multiple myeloma (MM) cell line KM(3) and its possible mechanism, cell viability was counted by trypan-blue exclusion, apoptosis was detected by morphology and DNA ladder; cell cycle was assayed by flow cytometry (FCM), telomerase activity was determined by semi-quantitative telomeric repeat amplification protocol (TRAP)-reverse transcription polymerase chain reaction (RT-PCR)-enzyme linked immunosorbent assay (ELISA), while the expression of hTERT mRNA in transcriptional level was measured by using RT-PCR. The results showed that arsenic trioxide inhibited the growth and viability of KM(3) cell and induced apoptosis; cell cycle was arrested in G(2) phase; arsenic trioxide could inhibit telomerase activity, which consisted with the downtrend of hTERT mRNA expression. In conclusion, down-regulation of telomerase activity and hTERT may play an important role in the apoptosis of MM cell line KM(3) induced by arsenic trioxide.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óxidos
/
Patologia
/
Farmacologia
/
Arsenicais
/
RNA Mensageiro
/
Ciclo Celular
/
Telomerase
/
Linhagem Celular Tumoral
/
Proteínas de Ligação a DNA
/
Tratamento Farmacológico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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