Effects of freeze-drying process on polymerized human placenta hemoglobin / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1052-1057, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-352115
ABSTRACT
The present study was aimed to investigate the influence of freeze-drying on the quality of polymerized human placenta hemoglobin (PolyPHb). The PolyPHb solution was freeze-drying under suitable conditions. Hemoglobin concentration, methemoglobin (MetHb) content, UV spectrum, Fe3 content, oxygen-carrying capacity, pH, the average molecular weight and its distribution, circular dichroism, oxygen equilibrium curve and other indicators were measured before and after freeze-drying. The appearance, residual water content, rehydration time of the lyophilized product were also evaluated. The results showed that there was no significant difference on all the indicators measured above, which indicated that freeze-drying process had no effect on the physical and chemical properties of PolyPHb, as well as on its biological activity. Therefore, the properties of PolyPHb were stable during this freeze-drying process and could be preserved after such freeze-drying process.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
Substitutos Sanguíneos
/
Hemoglobinas
/
Metemoglobina
/
Química
/
Polimerização
/
Liofilização
/
Métodos
Limite:
Feminino
/
Humanos
/
Gravidez
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS