Regulation of chondrosarcoma cell growth using synthesized hydrogels with different electric charges / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 782-788, 2013.
Article
em Zh
| WPRIM
| ID: wpr-352166
Biblioteca responsável:
WPRO
ABSTRACT
To develop standard in vitro chondrosarcoma models, we synthesized three hydrogels (i. e., PDMAAm, PNaAMPS and PMETAC) and investigated the influence of Young's modulus, swelling ratio and electric charges on the behavior of chondrosarcoma cells seeded on the hydrogels, including morphology, adhesion and aggregation. Results showed that the morphology of chondrosarcoma cells at 6h was dependent on the charges of hydrogels; cells present spindle-shaped and round-shaped morphology on negative charged and neutral hydrogel, respectively, while no cells spreaded on positive charged hydrogel. Chondrosarcoma cells formed aggregates on neutral PDMAAm after further culture. The hydrogels can be synthesized easily and has the characteristics of ease at use with defined components, which holds great potential for developing standard chondrosarcoma models in vitro.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias Ósseas
/
Química
/
Condrossarcoma
/
Hidrogéis
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Eletricidade Estática
/
Metacrilatos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2013
Tipo de documento:
Article